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Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, and Caspase-11 protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.

Journal: Balkan Medical Journal

Article Title: Fucoxanthin Attenuates Bisphenol A-Induced Testicular Injury via NF-κB-Mediated Pyroptosis Inhibition

doi: 10.4274/balkanmedj.galenos.2026.2025-11-268

Figure Lengend Snippet: Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, and Caspase-11 protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.

Article Snippet: Caspase-11 (NB120-10454) , Novus Biologicals (Littleton, USA).

Techniques: In Vivo, Quantitative RT-PCR, Immunofluorescence, Staining, Immunohistochemistry, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Binding Assay